Fermt3^tm1.1Efp
Targeted Allele Detail

Summary

• Symbol: Fermt3^tm1.1Efp
• Name: fermitin family member 3; targeted mutation 1.1, Edward F Plow
• MGI ID: MGI:5705846
• Synonyms: K3KI
• Gene: Fermt3 Location: Chr19:6976326-6996837 bp, - strand Genetic Position: Chr19, 5.13 cM
• Alliance: Fermt3^tm1.1Efp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227094
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: Site-directed mutagenesis mutated CAATGG into GCCGCC in exon 14, which led to a substitution of Q<597>W<598>/AA. An FRT-flanked neomycin cassette in intron 13 was removed via Flp-mediated recombination. Western blot analysis revealed normal expression levels of encoded protein in washed platelets from mutant mice. Coimmunoprecipitation experiments revealed a ~85% reduction in the association between integrin alphaIIbbeta3 and mutant protein in platelet lysates. (J:227094)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fermt3 Mutation: 31 strains or lines available

References

• Original: J:227094 Xu Z, et al., Direct interaction of kindlin-3 with integrin alphaIIbbeta3 in platelets is required for supporting arterial thrombosis in mice. Arterioscler Thromb Vasc Biol. 2014 Sep;34(9):1961-7
• All: 6 reference(s)