Foxh1^{tm3.1(Foxh1*)Cvw}
Targeted Allele Detail

Summary

• Symbol: Foxh1^{tm3.1(Foxh1*)Cvw}
• Name: forkhead box H1; targeted mutation 3.1, Christopher VE Wright
• MGI ID: MGI:5705374
• Synonyms: Foxh1^mEH1
• Gene: Foxh1 Location: Chr15:76552425-76554148 bp, - strand Genetic Position: Chr15, 36.24 cM
• Alliance: Foxh1^{tm3.1(Foxh1*)Cvw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217681
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, RMCE-ready)
• Mutations: Insertion, Nucleotide substitutions
• Foxh1^{tm3.1(Foxh1*)Cvw} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Foxh1 (MGI:1347465)

• Mutation details: Recombinase mediated cassette exchange with Foxh1^tm1Cvw inserted a FRT flanked selection cassette and a Foxh1 locus with a single amino acid mutation in the EH1 domain (FSIKSLLG to ESIKSLLG) and an N-terminal FLAG tag. This mutation disrupts interactions of the EH1 domain with the WD-repeat domain of Groucho related co-repressor proteins. Flp mediated recombination removed the selection cassette. (J:217681)

Expression

In Mice Carrying this Mutation:

17 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxh1 Mutation: 34 strains or lines available

References

• Original: J:217681 Halstead AM, et al., Disrupting Foxh1-Groucho interaction reveals robustness of nodal-based embryonic patterning. Mech Dev. 2015 May;136:155-65
• All: 1 reference(s)