Igh-J^tm1.1Crey
Targeted Allele Detail

Summary

• Symbol: Igh-J^tm1.1Crey
• Name: immunoglobulin heavy chain, joining region; targeted mutation 1.1, Claude-Agnes Reynaud
• MGI ID: MGI:5705150
• Synonyms: Tg-G
• Gene: Igh-J Location: unknown Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221629
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: A targeting vector inserted into the hypermutation site 43 bp downstream of segment 4 a 93 bp oligonucleotide, consisted of 10 repeats of a 9-mer sequence, TATTATTAA, with 4 repeats flanking 3 Gs separated by the same 9-mer sequence (oligo-G). Cre-mediated recombination removed the floxed neomycin resistance cassette. (J:221629)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh-J Mutation: 12 strains or lines available

References

• Original: J:221629 Zivojnovic M, et al., Somatic hypermutation at A/T-rich oligonucleotide substrates shows different strand polarities in Ung-deficient or -proficient backgrounds. Mol Cell Biol. 2014 Jun;34(12):2176-87
• All: 1 reference(s)