Ndnf^{tm1.1(folA/cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Ndnf^{tm1.1(folA/cre)Hze}
• Name: neuron-derived neurotrophic factor; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5702713
• Synonyms: Ndnf-IRES2-dgCre, Ndnf-IRES2-dgCre-D
• Gene: Ndnf Location: Chr6:65648595-65689307 bp, + strand Genetic Position: Chr6, 30.29 cM
• Alliance: Ndnf^{tm1.1(folA/cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227758
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: trimethoprim
• Mutation: Insertion
• Ndnf^{tm1.1(folA/cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ndnf (MGI:1915419)

• Mutation details: The targeting vector containa, from 5' to 3', a partial intron 2 through intron 3 sequence containing an FRT3 site in intron 3, a partial exon 4 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence) that is in-frame with the coding sequence, a dgCre fusion gene (described below) that is in-frame with the coding sequence, a bovine growth hormone polyA signal, an AttB site, a PGK-Neo-polyA cassette, an FRT5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. The dgCre fusion gene is an enhanced green fluorescent protein/Cre recombinase fusion gene with an N terminal fusion of the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. PhiC31 integrase-mediated recombination removed the AttB/AttP-flanked sequences. Although designed to be trimethoprim-inducible, the donating investigator reports the cre expression/activity pattern looks the same before and after TMP induction. No EGFP is detected with or without induction. (J:227758)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ndnf (mouse) Summary of all recombinase alleles driven by Ndnf. Alliance mouse Ndnf gene page
User Notes:	Additional information submitted by the research community Additional information submitted by the research community Although designed to be trimethoprim-inducible, the donating investigator reports the cre expression/activity pattern looks the same before and after TMP induction. Specifically, cre recombinase activity is specific and efficient (largely recapitulates the endogenous Ndnf expression pattern). Cre recombinase is expressed in layer 1 of cortex and in restricted populations within olfactory areas, hippocampal formation, striatum and midbrain. Cre recombinase expression is also found in vascular cells throughout the brain.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ndnf Mutation: 28 strains or lines available

References

• Original: J:227758 Tasic B, et al., Adult mouse cortical cell taxonomy revealed by single cell transcriptomics. Nat Neurosci. 2016 Feb;19(2):335-46
• All: 15 reference(s)