Thrb^tm3.1Df
Targeted Allele Detail

Summary

• Symbol: Thrb^tm3.1Df
• Name: thyroid hormone receptor beta; targeted mutation 3.1, Douglas Forrest
• MGI ID: MGI:5701356
• Synonyms: Thrb^b1
• Gene: Thrb Location: Chr14:4431611-4809435 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A3
• Alliance: Thrb^tm3.1Df page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222168
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s), Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Neomycin resistance and lacZ cassettes were targeted into a beta1 isoform-specific coding exon removing 84 codons and the splice donor of the exon. This mutation leaves the beta2 isoform-specific exon intact. Cre-mediated recombination removed the selection cassette. Expression of beta-galactosidase is detected in the cortex and medulla of the adrenals, with expression undetectable by P35 in males but persisting into adulthood in females. RT-PCR confirmed the absence of the beta1 isoform, but presence of the beta2 isoform, in P5 cochlea and P21 pituitary. (J:222168, J:227423)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thrb Mutation: 39 strains or lines available

References

• Original: J:222168 Huang CC, et al., A Novel Population of Inner Cortical Cells in the Adrenal Gland That Displays Sexually Dimorphic Expression of Thyroid Hormone Receptor-beta1. Endocrinology. 2015 Jun;156(6):2338-48
• All: 3 reference(s)