Snca^tm1.2Vlb
Targeted Allele Detail

Summary

• Symbol: Snca^tm1.2Vlb
• Name: synuclein, alpha; targeted mutation 1.2, Vladimir L Buchman
• MGI ID: MGI:5700504
• Synonyms: Snca^{delta floxed}
• Gene: Snca Location: Chr6:60708559-60806839 bp, - strand Genetic Position: Chr6, 29.15 cM
• Alliance: Snca^tm1.2Vlb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227400
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector containing a FRT site-flanked neo cassette and a loxP site was inserted downstream of exon 2 (the first coding exon) of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed. Germline, Cre-mediated recombination removed exon 2. Western blot analysis of neural tissues confirmed that no protein is expressed. (J:227400)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Snca Mutation: 34 strains or lines available

References

• Original: J:227400 Ninkina N, et al., A novel resource for studying function and dysfunction of alpha-synuclein: mouse lines for modulation of endogenous Snca gene expression. Sci Rep. 2015;5:16615
• All: 6 reference(s)