Pvalb^{tm2(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Pvalb^{tm2(cre/ERT2)Zjh}
• Name: parvalbumin; targeted mutation 2, Z Josh Huang
• MGI ID: MGI:5700403
• Synonyms: PV-2A-CreEr, PV-CreER
• Gene: Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
• Alliance: Pvalb^{tm2(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235123
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Pvalb^{tm2(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pvalb (MGI:97821)

• Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), followed by a frt-flanked neo cassette, upstream of the stop codon. (J:235123)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pvalb (mouse) Summary of all recombinase alleles driven by Pvalb. Comparative matrix view of recombinase activities Alliance mouse Pvalb gene page
User Notes:	Additional information submitted by the research community Additional information submitted by the research community The donating investigator reports that this strain is superior to Pvalb^tm1(cre/ERT2)Zjh (Stock No. 010777) in which CreERT2 is inserted into the initiation codon of the Pvalb gene, creating a knockin/knockout allele.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pvalb Mutation: 29 strains or lines available

References

• Original: J:235123 He M, et al., Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex. Neuron. 2016 Sep 21;91(6):1228-1243
• All: 3 reference(s)