Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc
Transgene Detail
|
Symbol: |
Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc |
Name: |
transgene insertion 26, Centro Nacional de Biotecnologia |
MGI ID: |
MGI:5699548 |
Synonyms: |
Tg(CAMK2A-KCNIP3*-lacZ)26Cnbc, Tg(CAMK2A-KCNIP3*-lacZ)JN26Cnbc, Tg(hCaMKIIalpha-hEFmDREAM-IRES-lacZ)JN26Cnbc |
Transgene: |
Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc Location: unknown
|
Alliance: |
Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc page
|
|
|
Transgene Type: |
|
Transgenic (Dominant negative, Inserted expressed sequence, Reporter) |
Mutation: |
|
Insertion
|
|
|
Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
KCNIP3 (30818) |
|
Transgene expresses a Ca2+ insensitive mutant protein ("EFmDREAM"). |
|
|
|
Mutation details: This transgene contains the human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A) promoter driving expression of a cDNA encoding human Kv channel interacting protein 3, calsenilin (KCNIP3; also called downstream regulatory element antagonist modulator (DREAM)) with two amino acid substitutions each at EF hands 2, 3 and 4 (EFmDREAM). The cDNA is followed by an internal ribosomal entry site (IRES) and a lacZ reporter gene. The amino acid substitutions render the mutant protein insensitive to Ca2+; it thus can block the Ca2+-dependent derepression function of endogenously expressed KCNIP3 and other Kv channel-interacting proteins. Line 26 is reported to exhibit "higher and clearer expression" in the telencephalon than line 1 or line 33. Distribution in line 26 is reported to be restricted to telencephalic neurons with trigeminal neurons devoid of noticeable expression.
(J:82809)
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
Original: |
J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013; |
All: |
2 reference(s) |
|