Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc
Transgene Detail

Summary

• Symbol: Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc
• Name: transgene insertion 26, Centro Nacional de Biotecnologia
• MGI ID: MGI:5699548
• Synonyms: Tg(CAMK2A-KCNIP3*-lacZ)26Cnbc, Tg(CAMK2A-KCNIP3*-lacZ)JN26Cnbc, Tg(hCaMKIIalpha-hEFmDREAM-IRES-lacZ)JN26Cnbc
• Transgene: Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc Location: unknown
• Alliance: Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAMK2A-KCNIP3*,-lacZ)26Cnbc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  KCNIP3 (30818)

  Kcnip3

  (MGI:1929258)

  Transgene expresses a Ca2+ insensitive mutant protein ("EFmDREAM").

• Mutation details: This transgene contains the human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A) promoter driving expression of a cDNA encoding human Kv channel interacting protein 3, calsenilin (KCNIP3; also called downstream regulatory element antagonist modulator (DREAM)) with two amino acid substitutions each at EF hands 2, 3 and 4 (EFmDREAM). The cDNA is followed by an internal ribosomal entry site (IRES) and a lacZ reporter gene. The amino acid substitutions render the mutant protein insensitive to Ca2⁺; it thus can block the Ca2⁺-dependent derepression function of endogenously expressed KCNIP3 and other Kv channel-interacting proteins. Line 26 is reported to exhibit "higher and clearer expression" in the telencephalon than line 1 or line 33. Distribution in line 26 is reported to be restricted to telencephalic neurons with trigeminal neurons devoid of noticeable expression. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 2 reference(s)