Gt(ROSA)26Sor^{tm6(CAG-Rspo3)Nki}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm6(CAG-Rspo3)Nki}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 6, The Netherlands Cancer Institute
• MGI ID: MGI:5697338
• Synonyms: Rspo3^inv, Rspo3inv^f
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm6(CAG-Rspo3)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:309330
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The targeting construct inserted into the locus a CAGGS promoter, loxP site, lox511 site, an inverted Rspo3 sequence, loxP site, lox511 site, polyadenylation sequence and FRT-flanked puromycin resistance cassette. Cre-mediated recombination is needed to restore correct orientation of the coding sequence under control of the upstream ubiquitous CAGGS promoter. (J:309330)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:309330 Hilkens J, et al., RSPO3 expands intestinal stem cell and niche compartments and drives tumorigenesis. Gut. 2017;66(6):1095-1105
• All: 5 reference(s)