Bps1^CcS4/Dem
QTL Variant Detail

Summary

• QTL variant: Bps1^CcS4/Dem
• Name: Bordetella pertussis susceptibility 1; CcS4/Dem
• MGI ID: MGI:5694096
• QTL: Bps1 Location: unknown Genetic Position: Chr12, Syntenic

Variant origin

• Strain of Specimen: CcS4/Dem

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:95621

Two different crosses involving recombinant congenic strains CcS-4/Dem and HcB-28/Dem were used to identify loci linked to susceptibility to infection with the bacteria Bordetella pertussis. CcS-4/Dem is derived from donor strain STS/A and background strain BALB/cHeJ whereas HcB-28/Dem is derived from donor strain C57BL/10ScSnA and background strain C3H/DiSnA. CcS-4/Dem carries STS/A-derived donor DNA in 8 segments over 7 chromosomes and HcB-28/Dem carries C57BL/10ScSnA-derived donor DNA in 8 segments over 7 chromosomes. 211 (CcS-4/Dem x BALB/cHeJ)F2 animals and 232 (HcB-28/Dem x C3H/DiSnA)F2 animals were generated. Only female mice were used in the analysis. Animals were inoculated with B. pertussis and after 1 week the number of bacteria found in the lungs was assessed. Recombinant congenic strain HcB-28/Dem is relatively resistant to B. pertussis infection.

In the (HcB-28/Dem x C3H/DiSnA)F2 population a locus exhibiting linkage to B. pertussis susceptibility was detected on mouse Chromosome 12. Peak linkage occurred near the SNP marker D12Rivm102 at 50 cM with a LOD score of 4.6. This locus is considered to be suggestive and is tentatively named Bps1 (Bordetella pertussis susceptibility 1). C3H/DiSnA-derived alleles confer recessive susceptibility toB. pertussis infection whereas C57BL/10ScSnA-derived alleles confer dominant resistance to B. pertussis infection. This locus explains 9.7% of the variance.

In the (CcS-4/Dem x BALB/cHeJ)F2 population an interacting locus pair exhibiting linkage to B. pertussis susceptibility mapped to 4.4 cM on mouse Chromosome 5 near D5Mit179 and to 50.3 cM on mouse Chromosome 11 near D11Mit122. This interacting locus pair reaches suggestive significance of LOD=2.92 and are tentatively named Bps2 and Bps3, respectively. Animals homozygous for BALB/cHeJ-derived alleles at both Bps2 and Bps3 exhibit the greatest susceptibility to B. pertussis. The effects of Bps2 and Bps3 explain approximately 7% of the variance.

References

• Original: J:95621 Banus HA, et al., Genetic control of Bordetella pertussis infection: identification of susceptibility loci using recombinant congenic strains of mice. Infect Immun. 2005 Feb;73(2):741-7
• All: 1 reference(s)