Tg(CAG-GCaMP2)2Mik
Transgene Detail

Summary

• Symbol: Tg(CAG-GCaMP2)2Mik
• Name: transgene insertion 2, Michael I Kotlikoff
• MGI ID: MGI:5693370
• Synonyms: pCAGGS-GCaMP2
• Transgene: Tg(CAG-GCaMP2)2Mik Location: unknown
• Alliance: Tg(CAG-GCaMP2)2Mik page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This 3.7 kbp transgene has a loxP site, the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), the GCaMP2 sequence (described below), a polyadenylation signal and a second loxP site. Founder line 2 was identified with high sensor expression.

  The genetically encoded calcium indicator GCaMP2 is a calcium-sensing molecule composed of a 35 aa polyHis plasmid leader sequence (RSET; essential for thermal stability), a 13 residue peptide of chicken smooth muscle myosin light chain kinase (M13), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), and a rat calmodulin DNA fragment (CaM; aa 2-148). The cpEGFP mutations result in increased brightness (D180Y and V93I), thermal stability (V163A and S175G) and GFP dimerization prevention (A206K). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)