Mki67^tm1.1Cle
Targeted Allele Detail

Summary

• Symbol: Mki67^tm1.1Cle
• Name: antigen identified by monoclonal antibody Ki 67; targeted mutation 1.1, Hans Clevers
• MGI ID: MGI:5661511
• Synonyms: Ki67^RFP, Mki67^tm1Cle
• Gene: Mki67 Location: Chr7:135291513-135318090 bp, - strand Genetic Position: Chr7, 81.32 cM, cytoband F3-F5
• Alliance: Mki67^tm1.1Cle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216072
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A TagRFP reporter was inserted in frame in the C terminus of the gene via homologous recombination. This mutation creates a C-terminal fusion ofTagRFP to the endogenous protein. Flp-mediated recombination removed the FRT-flanked neo cassette. Reporter expression is detected in actively dividing epithelial cells. (J:216072)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mki67 Mutation: 147 strains or lines available

References

• Original: J:216072 Basak O, et al., Mapping early fate determination in Lgr5+ crypt stem cells using a novel Ki67-RFP allele. EMBO J. 2014 Sep 17;33(18):2057-68
• All: 11 reference(s)