Atp2a2^tm1.1Raco
Targeted Allele Detail

Summary

• Symbol: Atp2a2^tm1.1Raco
• Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; targeted mutation 1.1, Richard A Cohen
• MGI ID: MGI:5660869
• Synonyms: SERCA 2 C674S, SKI
• Gene: Atp2a2 Location: Chr5:122591576-122640288 bp, - strand Genetic Position: Chr5, 62.38 cM, cytoband F
• Alliance: Atp2a2^tm1.1Raco page

Lack of blood vessels and smaller size of E8.5 Atp2a2^tm1.1Raco/Atp2a2^tm1.1Raco (S674/S674) embryos

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216004
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A mutated exon 14 with a TGT to TCC mutation resulting in a Cys674Ser substitution and an FRT flanked neo cassette in intron 14 were inserted via homologous recombination. Flp mediated recombination removed the neo cassette. This mutation removes half of the key thiol that is a target of ischemia induced S-glutathiolation. The presence of the C674S allele was confirmed by sequencing of both genomic DNA and cDNA from cardiac endothelial cells. (J:216004)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp2a2 Mutation: 76 strains or lines available

References

• Original: J:216004 Thompson MD, et al., Glutathione adducts on sarcoplasmic/endoplasmic reticulum Ca2+ ATPase Cys-674 regulate endothelial cell calcium stores and angiogenic function as well as promote ischemic blood flow recovery. J Biol Chem. 2014 Jul 18;289(29):19907-16
• All: 6 reference(s)