Gt(ROSA)26Sor^{tm5(ASPSCR1/TFE3)Mrc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm5(ASPSCR1/TFE3)Mrc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5, Mario R Capecchi
• MGI ID: MGI:5649273
• Synonyms: Rosa26-LSL-AT3
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm5(ASPSCR1/TFE3)Mrc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217462
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A cDNA clone was produced using RNA purified from a confirmed alveolar soft part sarcoma tumor. The cDNA contained the ASPCR1/TFE3 fusion product of the t(X;17) chromosomal translocation typical of these tumors. This cDNA was inserted downstream of a floxed STOP sequence consisting of the neomycin resistance gene and a polyadenylation transcription termination sequence and upstream of an IRES-EGFP. Both the fusion gene and EGFP will be translated form the same transcript following cre-mediated recombination to remove the STOP. (J:217462)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:217462 Goodwin ML, et al., Modeling alveolar soft part sarcomagenesis in the mouse: a role for lactate in the tumor microenvironment. Cancer Cell. 2014 Dec 8;26(6):851-62
• All: 2 reference(s)