F3^tm1Fct
Targeted Allele Detail

Summary

• Symbol: F3^tm1Fct
• Name: coagulation factor III, tissue factor; targeted mutation 1, Felix C Tanner
• MGI ID: MGI:5647876
• Synonyms: asTFKI
• Gene: F3 Location: Chr3:121517186-121528697 bp, + strand Genetic Position: Chr3, 52.94 cM
• Alliance: F3^tm1Fct page

Yolk sac abnormalities in F3^tm1Fct/F3^tm1Fct embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216519
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Modified isoform(s)) Mutations: Insertion, Intragenic deletion Mutation details: The open reading frame was replaced with one encoding the soluble alternatively spliced form and a loxP flanked neomycin resistance cassette. After homologous recombination into ES cells, the floxed neomycin resistance cassette was removed by transfection with a Cre expression plasmid. mRNA expression of the soluble form was 5.9-fold higher in homozygous mutant embryos relative to wild-type embryos, whereas mRNA expression of the full-length form was undetectable in homozygous mutant embryos. Western blot analysis revealed comparable protein expression of the soluble form in E9.5 homozygous mutant and wild-type embryos. (J:216519)

Generation of the F3^tm1Fct allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F3 Mutation: 26 strains or lines available

References

• Original: J:216519 Sluka SH, et al., Alternatively spliced tissue factor is not sufficient for embryonic development. PLoS One. 2014;9(5):e97793
• All: 1 reference(s)