Dohh^tm1.1Sbal
Targeted Allele Detail

Summary

• Symbol: Dohh^tm1.1Sbal
• Name: deoxyhypusine hydroxylase/monooxygenase; targeted mutation 1.1, Stefan Balabanov
• MGI ID: MGI:5646512
• Synonyms: Dohh^flox, Dohh^tm1bal, DOHH^tm2a/bal
• Gene: Dohh Location: Chr10:81220268-81224186 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dohh^tm1.1Sbal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214321
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 2 and an FRT flanked pgk-gb2-neo cassette and loxP site were inserted downstream of exon 4 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:214321)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dohh Mutation: 18 strains or lines available

References

• Original: J:214321 Sievert H, et al., A novel mouse model for inhibition of DOHH-mediated hypusine modification reveals a crucial function in embryonic development, proliferation and oncogenic transformation. Dis Model Mech. 2014 Aug;7(8):963-76
• All: 3 reference(s)