F13b^tm1Akic
Targeted Allele Detail

Summary

• Symbol: F13b^tm1Akic
• Name: coagulation factor XIII, beta subunit; targeted mutation 1, Akitada Ichinose
• MGI ID: MGI:5645820
• Gene: F13b Location: Chr1:139429440-139451490 bp, + strand Genetic Position: Chr1, 61.57 cM, cytoband F
• Alliance: F13b^tm1Akic page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222849
• Parent Cell Line: EB3/5 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment including all of exons 1 and 2 was replaced with a neo cassette via homologous recombination. Northern and western blot analysis confirmed the absence of mRNA and protein expression in homozygous mice. Expression of F13a1 was greatly reduced compared to wild-type in homozygous mice. (J:222849)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F13b Mutation: 37 strains or lines available

Notes

ES cell line EB3/5 is a mutant E14TG2a line carrying one inactivated allele of Pou5f1. J:222849 does not specify whether the line of mice created carries the inactivated Pou5f1 allele.

References

• Original: J:222849 Souri M, et al., Administration of factor XIII B subunit increased plasma factor XIII A subunit levels in factor XIII B subunit knock-out mice. Int J Hematol. 2008 Jan;87(1):60-8
• All: 3 reference(s)