Trp53^tm4Holl
Targeted Allele Detail

Summary

• Symbol: Trp53^tm4Holl
• Name: transformation related protein 53; targeted mutation 4, Monica Hollstein
• MGI ID: MGI:5645787
• Synonyms: Platform, PLF
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm4Holl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211918
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The mutation consists of the insertion of phiC31 integrase-specific sequences. The p53 platform consists of a pGK-neomycin selection marker flanked by two attP sites, which replaced sequences from the 5' end of intron 1 to the 5' end of intron 9 (exons 2-9) of the Trp53 gene. PhiC31 integrase- mediated cassette exchange (IMCe) can be achieved using this allele, with the insertion of mutant Trp53 constructs. (J:211918)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 232 strains or lines available

References

• Original: J:211918 Wei QX, et al., Rapid derivation of genetically related mutants from embryonic cells harboring a recombinase-specific Trp53 platform. Cell Cycle. 2011 Apr 15;10(8):1261-70
• All: 2 reference(s)