Per3tm2.1(PER3)Sarc
Targeted Allele Detail
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Symbol: |
Per3tm2.1(PER3)Sarc |
Name: |
period circadian clock 3; targeted mutation 2.1, Simon Archer |
MGI ID: |
MGI:5645738 |
Synonyms: |
Per35 |
Gene: |
Per3 Location: Chr4:151088109-151129122 bp, - strand Genetic Position: Chr4, 81.53 cM
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Alliance: |
Per3tm2.1(PER3)Sarc page
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Germline Transmission: |
Earliest citation of germline transmission:
J:222843
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Parent Cell Line: |
Bruce 4 (ES Cell)
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Strain of Origin: |
B6.Cg-Thy1a
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Allele Type: |
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Targeted (Humanized sequence, Inserted expressed sequence) |
Mutations: |
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Insertion, Intragenic deletion
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Per3tm2.1(PER3)Sarc expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
PER3 (8863) |
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Mutation details: The targeting construct contained a cDNA sequence containing sequences of wild-type (WT) mouse exons 1821, with a downstream phosphoglycerine kinase (PGK) promoter driving a neomycin (Neo) selection marker. The sequence was flanked by locus of loxP sites and followed by the human exon 18, containing the 5-repeat allele of the human variable-number tandem-repeat (VNTR). Cre mediated recombination removed the cDNA encoding sequences contained in exons 1821, allowing the expression of humanized exon 18 with the 5-repeat copy of the human variable-number tandem-repeat (VNTR) and endogenous mouse exons 19-21.
(J:222843)
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Original: |
J:222843 Hasan S, et al., A human sleep homeostasis phenotype in mice expressing a primate-specific PER3 variable-number tandem-repeat coding-region polymorphism. FASEB J. 2014 Jun;28(6):2441-54 |
All: |
1 reference(s) |
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