Per3^{tm1.1(PER3)Sarc}
Targeted Allele Detail

Summary

• Symbol: Per3^{tm1.1(PER3)Sarc}
• Name: period circadian clock 3; targeted mutation 1.1, Simon Archer
• MGI ID: MGI:5645736
• Synonyms: Per3⁴
• Gene: Per3 Location: Chr4:151088109-151129122 bp, - strand Genetic Position: Chr4, 81.53 cM
• Alliance: Per3^{tm1.1(PER3)Sarc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222843
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Per3^{tm1.1(PER3)Sarc} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PER3 (8863)

  Per3	(MGI:1277134)

• Mutation details: The targeting construct contained a cDNA sequence containing sequences of wild-type (WT) mouse exons 1821, with a downstream phosphoglycerine kinase (PGK) promoter driving a neomycin (Neo) selection marker. The sequence was flanked by locus of loxP sites and followed by the human exon 18, containing the 4-repeat allele of the human variable-number tandem-repeat (VNTR). Cre mediated recombination removed the cDNA encoding sequences contained in exons 1821, allowing the expression of humanized exon 18 with the 4-repeat copy of the human variable-number tandem-repeat (VNTR) and endogenous mouse exons 19-21. (J:222843)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Per3 Mutation: 47 strains or lines available

References

• Original: J:222843 Hasan S, et al., A human sleep homeostasis phenotype in mice expressing a primate-specific PER3 variable-number tandem-repeat coding-region polymorphism. FASEB J. 2014 Jun;28(6):2441-54
• All: 1 reference(s)