Igs7^{tm85.1(tetO-gltI/GFP*)Hze}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm85.1(tetO-gltI/GFP*)Hze}
• Name: intergenic site 7; targeted mutation 85.1, Hongkui Zeng
• MGI ID: MGI:5645698
• Synonyms: Ai85(TITL-iGluSnFr), Igs7
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm85.1(tetO-gltI/GFP*)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:219930
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The pTRE-LSL-iGluSnFr replacement vector was designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element, a modified Tet response element promoter, a floxed-STOP cassette, the iGluSnFr coding sequence (details below), a WPRE sequence (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. The iGluSnFr fusion gene is constructed from the Escherichia coli gltI gene and circularly permutated green fluorescent protein (cpGFP). Specifically, iGluSnFr has a 21 aa IgG K-leader sequence, the gltI aa 1-253, the LILV linker, the cpGFP (aa 148-239, an 18 bp flexible linker and aa 1-147), the L2NP linker, the gltI aa 254-279, an 11 aa c-Myc epitope and a 49 aa PDGR sequence (PDGR aa 513-561). iGluSnFr is an extremely fast and sensitive glutamate indicator with large signal-to-noise ratio, and kinetics appropriate for in vivo imaging. PhiC31-mediated recombination removed the AttB/AttP-flanked PGK-hygromycin-SV40polyA cassette and replaced it with the recombined AttB/AttP site (AttL). (J:219930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 64 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 5 reference(s)