Tardbp^tm1.1Jrob
Targeted Allele Detail

Summary

• Symbol: Tardbp^tm1.1Jrob
• Name: TAR DNA binding protein; targeted mutation 1.1, Janice Robertson
• MGI ID: MGI:5643696
• Synonyms: Taradbp^2lox
• Gene: Tardbp Location: Chr4:148696839-148711476 bp, - strand Genetic Position: Chr4, 78.77 cM
• Alliance: Tardbp^tm1.1Jrob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215201
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: BAC recombineering was used to insert a loxP site upstream of exon 2 and a neomycin resistance cassette flanked by FRT sites downstream of exon 3 followed by another loxP site. The neomycin selection cassette was removed via FLP-mediated recombination by breeding with Tg(ACTFLPe)9205Dym expressing mice, leaving exons 2 and 3 flanked by loxP sites. mRNA and protein expression of the adjacent Masp2 gene (in which the 2 last exons of Masp2 overlap with the 3' untranslated region) is reduced by about 50% in heterozygotes. Western blot analysis confirmed Taradbp protein expression in the brain, spinal cord, and liver tissue. (J:215201)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tardbp Mutation: 60 strains or lines available

References

• Original: J:215201 Dib S, et al., Gene targeting of mouse Tardbp negatively affects Masp2 expression. PLoS One. 2014;9(4):e95373
• All: 1 reference(s)