Tg(Rho*)TTM4Rieke
Transgene Detail

Summary

• Symbol: Tg(Rho*)TTM4Rieke
• Name: transgene insertion TTM4, Fred Rieke
• MGI ID: MGI:5641188
• Synonyms: Tg(Rho^{T336A/T340A/T342A})
• Transgene: Tg(Rho*)TTM4Rieke Location: unknown
• Alliance: Tg(Rho*)TTM4Rieke page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Tg(Rho*)TTM4Rieke expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sag (MGI:98227)

• Mutation details: The transgene expresses mouse Rho in which phosphorylating threonines 336, 340 and 342 have been converted to inactivating alanines. Serine-associated phosphorylation sites (S334, S338 and S343) remain functional. An A337V mutation was introduced to confer a linear epitope for mAb3A6, that allows specific recognition of transgenic versus endogenous opsin. (J:221472)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:221472 Azevedo AW, et al., C-terminal threonines and serines play distinct roles in the desensitization of rhodopsin, a G protein-coupled receptor. Elife. 2015;4
• All: 1 reference(s)