Tg(Rho*)5PRieke
Transgene Detail

Summary

• Symbol: Tg(Rho*)5PRieke
• Name: transgene insertion 5P, Fred Rieke
• MGI ID: MGI:5641078
• Synonyms: S343A
• Transgene: Tg(Rho*)5PRieke Location: unknown
• Alliance: Tg(Rho*)5PRieke page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Tg(Rho*)5PRieke expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Rho (MGI:97914)

• Mutation details: The transgene expresses mouse Rho carrying S334, T336, S338, T340, and T342 phosphorylation sites. The remaining phosphorylation sites in the Rho transgene, S343, is inactivated via a serine to alanine mutation. An A337V mutation was introduced to confer a linear epitope for mAb3A6, that allows specific recognition of transgenic versus endogenous opsin. (J:188325)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:188325 Mendez A, et al., Rapid and reproducible deactivation of rhodopsin requires multiple phosphorylation sites. Neuron. 2000 Oct;28(1):153-64
• All: 3 reference(s)