Rb1^tm3Fad
Targeted Allele Detail

Summary

• Symbol: Rb1^tm3Fad
• Name: RB transcriptional corepressor 1; targeted mutation 3, Frederick A Dick
• MGI ID: MGI:5638883
• Synonyms: Rb^deltaS, Rb^S
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm3Fad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217849
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A codon change from TTT to GCT introduced the amino acid substitution of alanine for phenylalanine at position 832 (F832A) in exon 24. A loxP-flanked PGK-Neo cassette was inserted downstream of the mutation and was removed via Cre-mediated recombination. This mutation abolishes binding to E2F1. (J:217849, J:249124)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 106 strains or lines available

References

• Original: J:217849 Coschi CH, et al., Haploinsufficiency of an RB-E2F1-Condensin II complex leads to aberrant replication and aneuploidy. Cancer Discov. 2014 Jul;4(7):840-53
• All: 2 reference(s)