Cers4^tm1.1Kwi
Targeted Allele Detail

Summary

• Symbol: Cers4^tm1.1Kwi
• Name: ceramide synthase 4; targeted mutation 1.1, Klaus Willecke
• MGI ID: MGI:5638668
• Synonyms: Cers4^-
• Gene: Cers4 Location: Chr8:4542863-4579603 bp, + strand Genetic Position: Chr8, 2.04 cM, cytoband A1.2
• Alliance: Cers4^tm1.1Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213606
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic region including exons 2 - 11 was replaced with an NLS-lacZ cassette and an FRT flanked neo cassette via homologous recombination. Flp mediated recombination removed the neo cassette. Ceramide synthase activity assays revealed a significant decrease in activity towards C20 acyl-CoA to ~10% in the epidermis and to ~20% in the dermis of homozygous mutant mice. (J:213606)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cers4 Mutation: 45 strains or lines available

References

• Original: J:213606 Ebel P, et al., Ceramide synthase 4 deficiency in mice causes lipid alterations in sebum and results in alopecia. Biochem J. 2014 Jul 1;461(1):147-58
• All: 2 reference(s)