Gt(ROSA)26Sor^tm1.1Fwa
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1.1Fwa
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Frederick W Alt
• MGI ID: MGI:5636852
• Synonyms: ROSA^I-SceI-GR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1.1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220884
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: triamcinolone acetonide
• Mutation: Insertion
• Mutation details: A floxed neo STOP cassette and an I-Scel-GR-IRES-tdTomato cassette were inserted into intron 1 via homologous recombination. The I-Scel-GR-IRES-tdTomato cassette encodes an I-Scel meganuclease-glucocorticoid receptor fusion protein that can be activated via triamcinolone acetonide. Cre mediated recombination removed the neo cassette. (J:220884)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1205 strains or lines available

References

• Original: J:220884 Chiarle R, et al., Genome-wide translocation sequencing reveals mechanisms of chromosome breaks and rearrangements in B cells. Cell. 2011 Sep 30;147(1):107-19
• All: 1 reference(s)