Igh^tm8Fwa
Targeted Allele Detail

Summary

• Symbol: Igh^tm8Fwa
• Name: immunoglobulin heavy chain complex; targeted mutation 8, Frederick W Alt
• MGI ID: MGI:5636850
• Synonyms: deltaSgamma1/I-Scel^a
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220884
• Parent Cell Line: EF1 (ES Cell)
• Strain of Origin: 129S/SvEv x C57BL/6

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: ES cells containing the targeting construct used to create Igh^tm2.1Fwa were retargeted to replace the floxed region with two I-Scel sites in inverted orientation flanking 500 bp of sequence that lacks class switch recombination activity. (J:220885)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

References

• Original: J:220885 Zarrin AA, et al., Antibody class switching mediated by yeast endonuclease-generated DNA breaks. Science. 2007 Jan 19;315(5810):377-81
• All: 3 reference(s)