Spag17^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Spag17^{tm1c(KOMP)Wtsi}
• Name: sperm associated antigen 17; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5636738
• Synonyms: Spag17^flox
• Gene: Spag17 Location: Chr3:99792722-100050638 bp, + strand Genetic Position: Chr3, 43.25 cM, cytoband F3
• Alliance: Spag17^{tm1c(KOMP)Wtsi} page
• IMPC: Spag17 gene page

Mutation origin

• Mutant Cell Line: EPD0234_6_G08
• Germline Transmission: Earliest citation of germline transmission: J:211752
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 99889093 of Chromosome 3 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 99889997. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp mediated recombination. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:211752)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spag17 Mutation: 108 strains or lines available

Notes

J:211752 used EPD0234_6_G08 ES cells.

References

• Original: J:211752 Teves ME, et al., Sperm-associated antigen-17 gene is essential for motile cilia function and neonatal survival. Am J Respir Cell Mol Biol. 2013 Jun;48(6):765-72
• All: 3 reference(s)