Slc12a5^tm1.1Msa
Targeted Allele Detail

Summary

• Symbol: Slc12a5^tm1.1Msa
• Name: solute carrier family 12, member 5; targeted mutation 1.1, Matti S Airaksinen
• MGI ID: MGI:5629854
• Synonyms: KCC2a-KO
• Gene: Slc12a5 Location: Chr2:164802766-164841651 bp, + strand Genetic Position: Chr2, 85.28 cM, cytoband G2-G3
• Alliance: Slc12a5^tm1.1Msa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214695
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced exon 1a. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of the a isoform and presence of b isoform in the brain. (J:214695)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc12a5 Mutation: 46 strains or lines available

References

• Original: J:214695 Markkanen M, et al., Distribution of neuronal KCC2a and KCC2b isoforms in mouse CNS. J Comp Neurol. 2014 Jun 1;522(8):1897-914
• All: 2 reference(s)