Stra8^{tm1(EGFP/cre/ERT2)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Stra8^{tm1(EGFP/cre/ERT2)Wtsi}
• Name: stimulated by retinoic acid gene 8; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5621007
• Synonyms: Stra8^{tm1(cre/ERT2)Hmgu}
• Gene: Stra8 Location: Chr6:34897098-34916279 bp, + strand Genetic Position: Chr6, 15.2 cM
• Alliance: Stra8^{tm1(EGFP/cre/ERT2)Wtsi} page

Mutation origin

• Mutant Cell Line: CEPD0059_3_C10
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMMTOOLS

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Stra8^{tm1(EGFP/cre/ERT2)Wtsi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Stra8 (MGI:107917)

• Mutation details: According to the allele image posted by EUCOMMTOOLS in the international knockout mouse consortium site (https://www.mousephenotype.org/imits/targ_rep), exons 4 and 5 are flanked by loxP sites and a cre/ERT2-EGRP cassette preceded by a single FRT site and followed by a Rox flanked puromycin selection cassette was inserted into the intron between exons 3 and 4. (J:155845)
• Inheritance: Not Specified

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Stra8 (mouse) Summary of all recombinase alleles driven by Stra8. Comparative matrix view of recombinase activities Alliance mouse Stra8 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stra8 Mutation: 35 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 2 reference(s)