Slc6a3^tm4.1(tTA)Xz
Targeted Allele Detail

Summary

• Symbol: Slc6a3^tm4.1(tTA)Xz
• Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 4.1, Xiaoxi Zhuang
• MGI ID: MGI:5619093
• Synonyms: DAT-PF-tTA
• Gene: Slc6a3 Location: Chr13:73684866-73726791 bp, + strand Genetic Position: Chr13, 40.1 cM
• Alliance: Slc6a3^tm4.1(tTA)Xz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218892
• Parent Cell Line: PRX-129S6 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: The following were inserted, in order, between the promoter and coding sequences of the endogenous gene: tTA-pA-STOP-"PF" [TetO-tTA] cassette-pA-loxP-Neo-loxP. The PF (positive feedback) amplification cassette enhances tTA expression following the removal of the STOP. Cre-mediated recombination removed the downstream floxed neo cassette. Transgene expression is eight-fold higher than endogenous alpha-synuclein expression in dopaminergic neurons. (J:218892)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc6a3 Mutation: 66 strains or lines available

References

• Original: J:218892 Chen L, et al., A53T human alpha-synuclein overexpression in transgenic mice induces pervasive mitochondria macroautophagy defects preceding dopamine neuron degeneration. J Neurosci. 2015 Jan 21;35(3):890-905
• All: 4 reference(s)