Zap70^tm5.1Mal
Targeted Allele Detail

Summary

• Symbol: Zap70^tm5.1Mal
• Name: zeta-chain (TCR) associated protein kinase; targeted mutation 5.1, Bernard Malissen
• MGI ID: MGI:5617469
• Synonyms: Zap70^OST
• Gene: Zap70 Location: Chr1:36800912-36821899 bp, + strand Genetic Position: Chr1, 15.41 cM, cytoband A4-C1
• Alliance: Zap70^tm5.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209882
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: Sequence for a OneSTrEPtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site were inserted in-frame at the 3 end of the gene coding sequence in exon 13. Cre-mediated self-excision removed the selection cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. (J:209882)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zap70 Mutation: 53 strains or lines available

References

• Original: J:209882 Roncagalli R, et al., Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub. Nat Immunol. 2014 Apr;15(4):384-392
• All: 1 reference(s)