Ins1^{tm2.1(cre/ERT2)Thor}
Targeted Allele Detail

Summary

• Symbol: Ins1^{tm2.1(cre/ERT2)Thor}
• Name: insulin I; targeted mutation 2.1, Bernard Thorens
• MGI ID: MGI:5614361
• Synonyms: Ins1^CreERT2, MIP1-CreERT2
• Gene: Ins1 Location: Chr19:52252735-52253453 bp, + strand Genetic Position: Chr19, 46.76 cM
• Alliance: Ins1^{tm2.1(cre/ERT2)Thor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217521
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Ins1^{tm2.1(cre/ERT2)Thor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ins1 (MGI:96572)

• Mutation details: A targeting construct was designed to insert a cre recombinase gene fused with a modified estrogen receptor gene (cre/ERT2) at the initiation codon of the gene with followed by a FRT-flanked neomycin resistance (neo) cassette, replacing the coding sequence. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:217521)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ins1 (mouse) Summary of all recombinase alleles driven by Ins1. Comparative matrix view of recombinase activities Alliance mouse Ins1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ins1 Mutation: 46 strains or lines available

References

• Original: J:217521 Thorens B, et al., Ins1(Cre) knock-in mice for beta cell-specific gene recombination. Diabetologia. 2015 Mar;58(3):558-65
• All: 11 reference(s)