Runx1^tm2.1Gss
Targeted Allele Detail

Summary

• Symbol: Runx1^tm2.1Gss
• Name: runt related transcription factor 1; targeted mutation 2.1, Gary S Stein
• MGI ID: MGI:5613812
• Synonyms: Runx1^{HTY350-352AAA}
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm2.1Gss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214025
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 8 was replaced with a modified one in which nucleotide substitutions (CACACCTAC to GCCGCGGCA) resulted in the amino acid substitution of three alanines for histidine, threonine and tyrosine at positions 350 to 352 (HTY350-352AAA). These mutations disrupt the subnuclear targeting/co-factor interaction domain. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted upstream of the modified exon 8. (J:214025)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 35 strains or lines available

References

• Original: J:214025 Dowdy CR, et al., A germline point mutation in Runx1 uncouples its role in definitive hematopoiesis from differentiation. Exp Hematol. 2013 Nov;41(11):980-991.e1
• All: 1 reference(s)