Cenpj^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cenpj^{tm1b(EUCOMM)Wtsi}
• Name: centromere protein J; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5609805
• Gene: Cenpj Location: Chr14:56764218-56812882 bp, - strand Genetic Position: Chr14, 28.28 cM
• Alliance: Cenpj^{tm1b(EUCOMM)Wtsi} page
• IMPC: Cenpj gene page

Mutation origin

• Mutant Cell Line: EPD0028_7_G05
• Germline Transmission: Earliest citation of germline transmission: J:208632
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 56792283 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56793168. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp mediated recombination. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cenpj Mutation: 44 strains or lines available

References

• Original: J:208632 Bazzi H, et al., Acentriolar mitosis activates a p53-dependent apoptosis pathway in the mouse embryo. Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):E1491-500
• All: 2 reference(s)