Pls1^tm1Fri
Targeted Allele Detail

Summary

• Symbol: Pls1^tm1Fri
• Name: plastin 1 (I-isoform); targeted mutation 1, Francisco Rivero
• MGI ID: MGI:5609592
• Gene: Pls1 Location: Chr9:95634695-95727359 bp, - strand Genetic Position: Chr9, 50.22 cM
• Alliance: Pls1^tm1Fri page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216723
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaces the second exon of the gene, which contains the start codon. Northern blot analysis indicates expression of low amounts of mRNA of slightly smaller size in intestinal mucosa of homozygotes. Western blot analysis of small intestine lysates indicates absence of protein in homozygotes and further analysis with pan-fimbrin polyclonal antiserum does not find any truncated protein. (J:216723)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pls1 Mutation: 38 strains or lines available

References

• Original: J:216723 Grimm-Gunter EM, et al., Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium. Mol Biol Cell. 2009 May;20(10):2549-62
• All: 4 reference(s)