Cnr1^tm1.1Kpm
Targeted Allele Detail

Summary

• Symbol: Cnr1^tm1.1Kpm
• Name: cannabinoid receptor 1; targeted mutation 1.1, Ken Mackie
• MGI ID: MGI:5607776
• Synonyms: S426A/S430A
• Gene: Cnr1 Location: Chr4:33924593-33948831 bp, + strand Genetic Position: Chr4, 16.28 cM
• Alliance: Cnr1^tm1.1Kpm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208116
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: An HA epitope sequence, two mutations that change serines 426 and 430 to alanines (S426A, S430A), and a neomycin phosphotransferase (NeoR) gene flanked by frt sequences were inserted via homologous recombination. Serines 426 and 430 are putative GRK phosphorylation sites that when mutated to alanines are nonphosphorylatable and result in a desensitization resistant form of Cnr1. The frt-flanked NeoR gene was removed via flp-mediated recombination. Southern blot confirmed expression of a 4.2 kb mutant fragment and PCR confirmed expression of the mutant mRNA. Western blot analysis confirmed that protein levels in the brain remain unchanged except for a 15% decrease in the cerebellum. (J:208116)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cnr1 Mutation: 43 strains or lines available

References

• Original: J:208116 Morgan DJ, et al., Mutation of Putative GRK Phosphorylation Sites in the Cannabinoid Receptor 1 (CB1R) Confers Resistance to Cannabinoid Tolerance and Hypersensitivity to Cannabinoids in Mice. J Neurosci. 2014 Apr 9;34(15):5152-63
• All: 3 reference(s)