Afdn^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Afdn^{tm1c(EUCOMM)Hmgu}
• Name: afadin, adherens junction formation factor; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5607284
• Synonyms: Mllt4^flox
• Gene: Afdn Location: Chr17:13980735-14126059 bp, + strand Genetic Position: Chr17, 8.95 cM, cytoband A2
• Alliance: Afdn^{tm1c(EUCOMM)Hmgu} page
• IMPC: Afdn gene page

Mutation origin

• Mutant Cell Line: HEPD0591_8_E01
• Germline Transmission: Earliest citation of germline transmission: J:215584
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 14028703 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 14029592. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele. (J:215584)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Afdn Mutation: 82 strains or lines available

References

• Original: J:215584 Gil-Sanz C, et al., Proliferative defects and formation of a double cortex in mice lacking Mltt4 and Cdh2 in the dorsal telencephalon. J Neurosci. 2014 Aug 6;34(32):10475-87
• All: 3 reference(s)