Ogfod1^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ogfod1^{tm1d(KOMP)Wtsi}
• Name: 2-oxoglutarate and iron-dependent oxygenase domain containing 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5607136
• Synonyms: Ogfod1^-
• Gene: Ogfod1 Location: Chr8:94763826-94794549 bp, + strand Genetic Position: Chr8, 46.08 cM
• Alliance: Ogfod1^{tm1d(KOMP)Wtsi} page
• IMPC: Ogfod1 gene page

Mutation origin

• Mutant Cell Lines: EPD0064_2_B12, EPD0064_2_C11
• Germline Transmission: Earliest citation of germline transmission: J:207410
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 94778264 of Chromosome 8 upstream of the exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 4 at position 94779080. Exon 4 is thus flanked by loxP sites. Flp mediated recombination removed the lacZ/neo cassette. Cre mediated recombination removed exon 4. (J:207410)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ogfod1 Mutation: 31 strains or lines available

Notes

Cell lines EPD0064_2_B12 and EPD0064_2_C11 were successfully used to make chimeric mice. Germline transmission was accomplished for both lines. J:207410

References

• Original: J:207410 Singleton RS, et al., OGFOD1 catalyzes prolyl hydroxylation of RPS23 and is involved in translation control and stress granule formation. Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4031-6
• All: 4 reference(s)