Prnp^tm1Wsj
Targeted Allele Detail

Summary

• Symbol: Prnp^tm1Wsj
• Name: prion protein; targeted mutation 1, Walker S Jackson
• MGI ID: MGI:5606060
• Synonyms: Ki-Prnp-GFP
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^tm1Wsj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215179
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The third exon was replaced with an enhanced GFP gene. Quantitative real-time PCR confirmed absence of Prnp mRNA in homozygotes and lower levels, about 50-60% that of wild-type, in heterozygotes. GFP expression was confirmed in various regions of the brain and in astrocytes. (J:215179)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 150 strains or lines available

References

• Original: J:215179 Jackson WS, et al., Translation of the prion protein mRNA is robust in astrocytes but does not amplify during reactive astrocytosis in the mouse brain. PLoS One. 2014;9(4):e95958
• All: 1 reference(s)