Ptentm1.1Whs
Targeted Allele Detail
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Symbol: |
Ptentm1.1Whs |
Name: |
phosphatase and tensin homolog; targeted mutation 1.1, Wen H Shen |
MGI ID: |
MGI:5605962 |
Synonyms: |
PTENdeltaC, Ptentm1.1Yxy |
Gene: |
Pten Location: Chr19:32734977-32803560 bp, + strand Genetic Position: Chr19, 28.14 cM
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Alliance: |
Ptentm1.1Whs page
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Germline Transmission: |
Earliest citation of germline transmission:
J:211716
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Parent Cell Line: |
Other (see notes) (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Not Applicable) |
Mutations: |
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Insertion, Single point mutation
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Mutation details: This mutation was created by generating targeting constructs containing a 5' homology region with Pten exons 4-5, a neo cassette flanked by two loxP sites (LoxP- Neo-LoxP), and a 3' homology region containing exon 6 with the point mutation (c.565A > T) preceded by a FLAG tag. After homologous recombination in ES cells and injection into C57BL/6 blastocysts, the resulting chimeras were bred with EIIA-Cre transgenic female mice to delete the loxP-flanked neomycin resistance gene (neo) from the targeted allele. The C-terminal truncated Pten protein, is expressed in various tissues including kidney, lung, uterus and heart.
(J:211716)
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The mutation was generated in an unspecified ES cell line derived from C57BL/6 and 129S6/SvEvTac. J:211716)
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Original: |
J:211716 Sun Z, et al., PTEN C-terminal deletion causes genomic instability and tumor development. Cell Rep. 2014 Mar 13;6(5):844-54 |
All: |
1 reference(s) |
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