Tg(Chat-tau/EGFP)#Svj
Transgene Detail

Summary

• Symbol: Tg(Chat-tau/EGFP)#Svj
• Name: transgene insertion, Sukumar Vijayaraghavan
• MGI ID: MGI:5605267
• Synonyms: ChAT-tauGFP
• Transgene: Tg(Chat-tau/EGFP)#Svj Location: unknown
• Alliance: Tg(Chat-tau/EGFP)#Svj page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The bacterial artificial chromosome RP23-431D9, which contains the entire mouse choline acetyltransferase gene, was modified by insertion at the initiating ATG codon, in exon 3, of a DNA sequence encoding a tau/enhanced green fluorescent protein (tau/EGFP) fusion protein followed by a triple polyadenylation signal and a kanamycin resistance gene (aphAI). Detailed comparison of EGFP fluorescence/immunohistochemical staining and endogenous CHAT expression, visualized immunohistochemically, demonstrates their almost complete co-localization throughout the brain and peripheral nervous system; as anticipated, fusion to tau localizes EGFP to axons. (J:176375)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:176375 Grybko MJ, et al., A transgenic mouse model reveals fast nicotinic transmission in hippocampal pyramidal neurons. Eur J Neurosci. 2011 May;33(10):1786-98
• All: 13 reference(s)