Gt(ROSA)26Sor^{tm75.1(CAG-tdTomato*)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm75.1(CAG-tdTomato*)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 75.1, Hongkui Zeng
• MGI ID: MGI:5603432
• Synonyms: Ai75, Ai75(RCL-nls tdT), Ai75(RCL-nT)-D, Ai75(Rosa-CAG-LSL-nT)-D
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm75.1(CAG-tdTomato*)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268846
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-pCAG-LSL-ntdTomato-WPRE-bGHpA-AttB-PGK-neo-polyA-AttP targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the ntdTomato coding sequence (nT; a tdTomato fluorescent protein sequence with amnio-terminal addition of a 30bp fragment encoding a nuclear localization signal), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance mRNA transcript stability), a bovine growth hormone polyA signal, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, and an AttP site. PhiC31o-mediated recombination removed the AttB/AttP-flanked PGK-Neo-polyA cassette and replaced it with the recombined AttB/AttP site (AttL). (J:268846)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:268846 Quina LA, et al., Specific connections of the interpeduncular subnuclei reveal distinct components of the habenulopeduncular pathway. J Comp Neurol. 2017 Aug 15;525(12):2632-2656
• All: 21 reference(s)