Hsbp1^tm1Miv
Targeted Allele Detail

Summary

• Symbol: Hsbp1^tm1Miv
• Name: heat shock factor binding protein 1; targeted mutation 1, Nahid F Mivechi
• MGI ID: MGI:5588201
• Gene: Hsbp1 Location: Chr8:120071277-120075668 bp, + strand Genetic Position: Chr8, 67.54 cM
• Alliance: Hsbp1^tm1Miv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:206878
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 1 following the ATG codon, exon 2, and intron 1 were replaced with a cassette containing an EGFP reporter gene, the thymidine kinase promoter with a simian virus 40 polyA signal and a PGK-neomycin gene flanked by two loxP sites, creating an out-of-frame deletion. Western blot confirmed absence of protein expression. EGFP expression is driven by the Hsbp1 promoter and is detected at E0.5 and E1.5 in blastocysts and during later stages (E8.5-10.5) in heterozygotes. (J:206878)

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsbp1 Mutation: 10 strains or lines available

References

• Original: J:206878 Eroglu B, et al., An essential role for heat shock transcription factor binding protein 1 (HSBP1) during early embryonic development. Dev Biol. 2014 Feb 15;386(2):448-60
• All: 1 reference(s)