A^tm1.1Arte
Targeted Allele Detail

Summary

• Symbol: A^tm1.1Arte
• Name: nonagouti; targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:5585618
• Gene: a Location: Chr2:154792519-154892932 bp, + strand Genetic Position: Chr2, 76.83 cM
• Alliance: A^tm1.1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213411
• Parent Cell Line: ART B6-3, B6-3 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 14.7 kb retrotransposon insertion in intron 1 of the a (nonagouti) allele, which functionally inactivates the gene, was replaced by a neomycin and puromycin resistance cassette flanked by frt sequences. The selection cassette was excised by flp recombinase, leaving a single frt site in the intron. (J:213411)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any a Mutation: 463 strains or lines available

Notes

Germline deletion of the frt-flanked selection cassette was achieved by crossing mice bearing the selection cassette (in place of the retrotransposon insertion) to C57BL/6NTac-Tg(CAG-flpe)2Arte "flp-deleter" mice. (J:213411)

References

• Original: J:213411 Zevnik B, et al., C57BL/6N albino/agouti mutant mice as embryo donors for efficient germline transmission of C57BL/6 ES cells. PLoS One. 2014;9(3):e90570
• All: 1 reference(s)