Sec23b^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sec23b^{tm1d(EUCOMM)Wtsi}
• Name: SEC23 homolog B, COPII coat complex component; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5583845
• Gene: Sec23b Location: Chr2:144398165-144432673 bp, + strand Genetic Position: Chr2, 71.02 cM, cytoband H1
• Alliance: Sec23b^{tm1d(EUCOMM)Wtsi} page
• IMPC: Sec23b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213188
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 144408239 of Chromosome 2 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 144409359. The critical exons 5 and 6 are thus flanked by loxP sites. Flp recombinase expression removed the reporter and selection cassettes. Cre-mediated recombination removed exons 5 and 6. (J:213188)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sec23b Mutation: 50 strains or lines available

References

• Original: J:213188 Khoriaty R, et al., Absence of a Red Blood Cell Phenotype in Mice with Hematopoietic Deficiency of SEC23B. Mol Cell Biol. 2014 Oct 1;34(19):3721-34
• All: 3 reference(s)