Cgas^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cgas^{tm1d(EUCOMM)Hmgu}
• Name: cyclic GMP-AMP synthase; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5578170
• Synonyms: cGAS KO, Mb21d1^-
• Gene: Cgas Location: Chr9:78337808-78350519 bp, - strand Genetic Position: Chr9, 43.65 cM
• Alliance: Cgas^{tm1d(EUCOMM)Hmgu} page
• IMPC: Cgas gene page

Mutation origin

• Mutant Cell Line: HEPD0516_3_C04
• Germline Transmission: Earliest citation of germline transmission: J:207826
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 78344357 of Chromosome 9 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 78345237. The critical exon 2 is thus flanked by loxP sites. Flp-mediated recombination removed the beta-geo cassette and left exon 2 floxed. Cre-mediated recombination removed exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:207826)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cgas Mutation: 26 strains or lines available

References

• Original: J:207826 Schoggins JW, et al., Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity. Nature. 2014 Jan 30;505(7485):691-5
• All: 143 reference(s)