Hprt1^{tm1(TG-cre)1Sasr}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(TG-cre)1Sasr}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Samia Selmi-Ruby
• MGI ID: MGI:5576753
• Synonyms: Tg(TG-cre)1Sasr, Thyr-Cre
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(TG-cre)1Sasr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208718
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Hprt1^{tm1(TG-cre)1Sasr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  cattle	TG (280706)

• Mutation details: A construct with a 2 kb bovine thryoglobulin (TG) regulatory region, intron II of the rabbit beta globin gene, nucleus-targeted cre recombinase sequence, and the SV40 polyA site was inserted into an Hprt destination vector which has a homology arm matching the 5' upstream region of Hprt and a 3' homology region containing proximal portions of the Hprt gene. Homologous recombination in ES cells with the proximal exon 3 deletion of Hprt resulted in integration of 1 copy of the transgene and restoration of a functional Hprt gene. (J:208718)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	TG (cattle) Summary of all recombinase alleles driven by TG. Comparative matrix view of recombinase activities

Expression

In Mice Carrying this Mutation:

62 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1274 strains or lines available

References

• Original: J:208718 Selmi-Ruby S, et al., The targeted inactivation of TRbeta gene in thyroid follicular cells suggests a new mechanism of regulation of thyroid hormone production. Endocrinology. 2014 Feb;155(2):635-46
• All: 1 reference(s)