Tg(Ube3a)1Mpan
Transgene Detail

Summary

• Symbol: Tg(Ube3a)1Mpan
• Name: transgene insertion 1, Matthew Anderson
• MGI ID: MGI:5576240
• Synonyms: FLAG-Ube3a
• Transgene: Tg(Ube3a)1Mpan Location: unknown
• Alliance: Tg(Ube3a)1Mpan page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ube3a)1Mpan expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ube3a (MGI:105098)

• Mutation details: The bacterial artificial chromosome (BAC) clone RP24-178G7 contains the entire ubiquitin protein ligase E3A locus (Ube3a) as well as ~63 kbp of 5' flanking sequence and ~21 kbp of 3' flanking sequence. DNA sequences encoding three FLAG epitope tags and two translational stop codons were inserted in-frame at the 3' coding/untranslated boundary of exon 12 of the gene on the BAC via homologous recombination/BAC recombineering. A single frt site remains just downstream of the 3xFLAG region. Two founder lines with independent integration sites were established; lines 1 and 2. Both founder lines exhibit the same expression pattern in the tissues examined (although line 2 may have slightly lower levels). Animals from line 1 were more extensively characterized. Ube3a expression was confirmed by Western blot of cortical lysates. (J:178319)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:178319 Smith SE, et al., Increased gene dosage of Ube3a results in autism traits and decreased glutamate synaptic transmission in mice. Sci Transl Med. 2011 Oct 5;3(103):103ra97
• All: 10 reference(s)